columns used in HPLC Fundamentals Explained
If the cell section’s pH is adequately acidic, the solutes are existing as neutral weak acids which might be extra soluble during the stationary section and just take lengthier to elute. Since the weak acid solutes do not need similar p-hydroxybenzoic acid (PH) on the nonpolar C18 column issue to the most analysis time of 6 min. The shaded region